Jeoung-Sook Shin, Ph.D.
Department of Microbiology & Immunology
Sandler Asthma Basic Research Center
University of California San Francisco
513 Parnassus Ave, HSE-201
San Francisco, CA 94143-0414
Jeoung-Sook Shin is an Associate Professor in the Department of Microbiology & Immunology. She completed her B.S. and M.S. in Chemistry at Seoul National University, Korea. She received her Ph.D. from Duke University and her postdoctoral training at Yale University as a Jane Coffin Childs Memorial Fund Postdoctoral Fellow.
The Shin laboratory is interested in understanding the molecular mechanism and function of dendritic cell-mediated antigen presentation. Dr. Shin has previously found that the antigenpresenting molecule MHCII is ubiquitinated by MARCH1 ubiquitin ligase in dendritic cells, and this ubiquitination mediates MHCII endocytosis and lysosomal degradation. Her laboratory has recently found that the costimulatory molecule CD86 is also ubiquitinated by MARCH1 and that this ubiquitination also mediates endocytosis and degradation of CD86. More recently, Dr. Shin’s laboratory has studied the functional role of this ubiquitination. This study indicates that MHCII ubiquitination is required for proper production of regulatory T cells (Tregs) in the thymus. Currently, Dr. Shin is investigating how MHCII ubiquitination contributes to Treg development and whether Tregs generated in MHCII ubiquitination-dependent manner are distinct in their repertoire and function.
The Shin laboratory is also interested in understanding the role of the high affinity IgE receptor FceRI expressed in dendritic cells. Although the role of FceRI in the pathogenesis of allergy is well known, its physiologic role remains unclear. Dr. Shin’s laboratory has recently found that FceRI is constitutively endocytosed and transported to the lysosomes in human dendritic cells and monocytes, and that this FceRI endolysosomal trafficking mediates cellular entry of circulating IgE contributing to serum IgE clearance. These findings suggest that FceRI expressed by dendritic cells and monocytes may play an important role in regulating serum IgE concentration in humans. Her laboratory is currently investigating whether unusually high blood IgE levels found in some human diseases is attributed to the alteration in FceRI endolysosomal trafficking that results circulating IgE not efficiently entering cells but accumulating in the blood.
Dr. Shin’s research programs are greatly benefited by many of the excellent core facilities supported by SABRE. Flow cytometry core is being used in a daily basis for most of the projects. Microscopy facility is helping in situ analysis of dendritic cells in human tissues and also the analysis of protein distribution inside dendritic cells. Mouse physiology core is being used to test the therapeutic potential of human IgE derivative that Dr. Shin has recently found to be capable of regulating immune stimulation.
- Shin, JS, Gao, Z, Abraham, SN. Involvement of cellular caveolae in bacterial entry into mast cells, Science. 289:785-8, 2000.
- Shin, JS, Abraham, SN. Glycosylphosphatidylinositol-anchored receptor-mediated bacterial endocytosis, FEMS Microbiol Lett.197: 131-8, 2001.
- Shin JS, Abraham SN. Caveolae as portals of entry for microbes. Microbes Infect. 3:755- 61, 2001.
- Shin JS, Abraham SN. Cell biology. Caveolae--not just craters in the cellular landscape. Science. 293:1447-8, 2001.
- Duncan, MJ, Shin JS, Abraham SN. Microbial entry through caveolae: variations on a theme. Cell Microbiol. 4: 783-91, 2002.
- Duncan, MJ, Lee, G, Shin, JS. Abraham, SN. Bacterial penetration of bladder epithelium through lipid rafts, J Biol Chem. 279: 18944-51, 2004.
- Shin, JS, Shelburne, CP, Jin, C, LeFurgey, EA, Abraham, SN. Harboring of particulat allergens within secretory compartments by mast cells following IgE/FcεRI-lipid raft mediated phagocytosis, J Immunol. 177:5791-5800, 2006.
- Shin, JS, Ebersold, M, Pypaert, M, Delamarre, L, Hartley, A, and Mellman, I. Surface expression of MHC class II in dendritic cells is controlled by regulated ubiquitination, Nature. 444:115-8, 2006.
- Bloom, O, Unternaehrer, JJ, Jiang, A, Shin, JS, Delamarre, L, Allen, P, and Mellman, I. Spinophilin participates in information transfer at immunological synapses, J Cell Biol. 181:203-11, 2008.
- Baravalle, G, Park, H, McSweeney, M, Ohmura-Hoshino, M, Matsuki, Y, Shin, JS. Ubiquitination of CD86 is a key mechanism in regulating antigen presentation by dendritic cells, J Immunology. 187:2966, 2011.
- Ma, JK, Platt, MY, Eastham-Anderson, J, Shin, JS*, and Mellman, I*. MHC class II distribution in dendritic cells and B cells is determined by ubiquitin chain length, PNAS. 109:8820, 2012 *Shin, JS and Mellman, contributed equally to this work.
- Oh, J, Wu, N, Baravalle, G, Cohn, B, Ma, J, Lo, B, Mellman, I, Ishido, S, Anderson, M, and Shin, JS. MARCH1-mediated MHCII ubiquitination promotes dendritic cell selection of natural regulatory T cells, J Exp Med. 210:1069, 2013.
- Greer, A, Wu, N, Putnam, A, Woodruff, P, and Shin, JS. Serum IgE clearance is facilitated by human FceRI internalization. J Clin Inves. 124:1187, 2014.
- Baravalle, G, Greer, A, LaFlam, TN, and Shin, JS. Antigen-conjugated human IgE induces antigen-specific T cell tolerance in a humanized mouse model, J Immunology. 192:3280, 2014.
- Eggel, A, Baravalle, G, Hobi,G, Kim, B, Buschor, P, Forrer, P, Shin, JS, Vogel, M, Stadler, BM, Dahinden, CA, and Jardetzky, TS. Accelerated dissociation of IgE:FceRI complexes by disruptive inhibitors actively desensitzes allergic effector cells, J of Allergy and Clinical Immunol. 133:1709, 2014.
- Greer, AM, Matthay, MA, Kukreja, J, Bhakta, NR, Nguyen, CP, Wolters, PJ, Woodruff, PG, Fahy, JV, and Shin, JS. Accumulation of BDCA1+ dendritic cells in interstitial fibrotic lung diseases and Th2-high asthma. PLoS ONE, Jun 9(6):e99084, 2014.
- Greer, AM and Shin, JS. The Role of FceRI expressed by dendritic cells and monocytes. Cellular and Molecular Life Sciences. 72:2349, 2015.
- Oh J, Shin, JS. Molecular mechanism and cellular function of MHCII ubiquitination. Immunological reviews. 266:134, 2015.